rabbit polyclonal antibody against human st3gal1  (Absolute Biotech Inc)

Journal: Cell Death & Disease

Article Title: Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer

doi: 10.1038/s41419-018-1101-0

Figure Lengend Snippet: ST3GAL1 expression and clinicopathological characteristics of 78 patients with ovarian cancer

Article Snippet: Primary antibodies included a Rabbit Polyclonal antibody against human ST3GAL1 (LS-C185763, LifeSpan BioSciences) at a dilution of 1:1000.

Techniques: Expressing, Histopathology

Journal: Cell Death & Disease

Article Title: Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer

doi: 10.1038/s41419-018-1101-0

Figure Lengend Snippet: a ST3GAL1 expression in 78 ovarian cancer tumor tissues and 15 relative normal tissues analyzed by qRT-PCR (**p < 0.01, Student’s t-test). b ST3GAL1 protein expression was analyzed by western blots in ovarian cancer tissues. c ST3GAL1 expression analyzed by immunohistochemistry in ovarian cancer tissues and normal tissues, respectively

Article Snippet: Primary antibodies included a Rabbit Polyclonal antibody against human ST3GAL1 (LS-C185763, LifeSpan BioSciences) at a dilution of 1:1000.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemistry

Journal: Cell Death & Disease

Article Title: Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer

doi: 10.1038/s41419-018-1101-0

Figure Lengend Snippet: a qRT-PCR analysis of ST3GAL1 expression in the ovarian cancer cell lines SKOV-3, OVCAR3, and A2780 and the normal ovarian cell line NOEC (*p < 0.05, **p < 0.01, and ***p < 0.001, one-way ANOVA). b Knockdown of ST3GAL1 (ST3GAL1 shRNA1#–3#) in SKOV-3 cells and qRT-PCR assessment of ST3GAL1 expression (*p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA). SKOV-3 cells were transfected with ST3GAL1-shRNA1# or shRNA2# and A2780 cells were transfected overexpression ST3GAL1 (ST3GAL1-OE). c–e Cell growth was determined by the CCK-8 assay (*p < 0.05, **p < 0.01, one-way ANOVA). f, g Transwell assay was used to detect cell migration and invasion (*p < 0.05, and **p < 0.01, one-way ANOVA)

Article Snippet: Primary antibodies included a Rabbit Polyclonal antibody against human ST3GAL1 (LS-C185763, LifeSpan BioSciences) at a dilution of 1:1000.

Techniques: Quantitative RT-PCR, Expressing, Knockdown, Transfection, Over Expression, CCK-8 Assay, Transwell Assay, Migration

Journal: Cell Death & Disease

Article Title: Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer

doi: 10.1038/s41419-018-1101-0

Figure Lengend Snippet: a, b SKOV-3 and A2780 cells were treated with or without 10 ng/mL TGF-β1, qRT-PCR analyzed ST3GAL1 mRNA expression (**p < 0.01, Student’s t-test). SKOV-3 cells treated with TGF-β1 or transfected with ST3GAL1-shRNA1# or shRNA2#, or both TGF-β1 and ST3GAL1-shRNA1# or shRNA2#, untransfected cells were used as a control. c, d E-cadherin, N-cadherin, and Vimentin protein expression were analyzed by western blots. A2780 cells treated with TGF-β1 or transfected with ST3GAL1-OE, or both TGF-β1 and ST3GAL1-OE, untransfected cells were used as a control. e, f E-cadherin, N-cadherin, and Vimentin protein expression were analyzed by western blots. (*p < 0.05, **p < 0.01, one-way ANOVA)

Article Snippet: Primary antibodies included a Rabbit Polyclonal antibody against human ST3GAL1 (LS-C185763, LifeSpan BioSciences) at a dilution of 1:1000.

Techniques: Quantitative RT-PCR, Expressing, Transfection, Control, Western Blot

Journal: Cell Death & Disease

Article Title: Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer

doi: 10.1038/s41419-018-1101-0

Figure Lengend Snippet: SKOV-3 cells treated with TGF-β1 or transfected with ST3GAL1-shRNA1# or shRNA2#, or both TGF-β1 and ST3GAL1-shRNA1# or shRNA2#, untransfected cells were used as a control. A2780 cells treated with TGF-β1 or transfected with ST3GAL1-OE, or both TGF-β1 and ST3GAL1-OE, untransfected cells were used as a control. a, b E-cadherin and N-cadherin protein expression were analyzed by cell immunofluorescence

Article Snippet: Primary antibodies included a Rabbit Polyclonal antibody against human ST3GAL1 (LS-C185763, LifeSpan BioSciences) at a dilution of 1:1000.

Techniques: Transfection, Control, Expressing, Immunofluorescence

Journal: Cell Death & Disease

Article Title: Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer

doi: 10.1038/s41419-018-1101-0

Figure Lengend Snippet: SKOV-3 cells treated with TGF-β1 or transfected with ST3GAL1-shRNA1# or shRNA2#, or both TGF-β1 and ST3GAL1-shRNA1# or shRNA2#, untransfected cells were used as a control. A2780 cells treated with TGF-β1 or transfected with ST3GAL1-OE, or both TGF-β1 and ST3GAL1-OE, untransfected cells were used as a control. a, b SKOV-3 and A2780 cells migration and invasion were analyzed by Transwell assay. *p < 0.05, and **p < 0.01

Article Snippet: Primary antibodies included a Rabbit Polyclonal antibody against human ST3GAL1 (LS-C185763, LifeSpan BioSciences) at a dilution of 1:1000.

Techniques: Transfection, Control, Migration, Transwell Assay

Journal: Cell Death & Disease

Article Title: Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer

doi: 10.1038/s41419-018-1101-0

Figure Lengend Snippet: a, b SKOV-3 and A2780 cells were treated with 0, 3.125, 6.25, 12.5, 25, 50, 100, 200, 400, and 800 ng/mL Taxol for 7 days, and the half maximal inhibitory concentration (IC 50) was detected. The IC50 of A2780 is 101 ng/mL and the IC50 of SKOV-3 cells is 147 ng/mL. c, d A colony formation assay of A2780 cells transfected with ST3GAL1 (ST3GAL1-OE) for overexpression. SKOV-3 cells transduced with shRNA1# or 2# for ST3GAL1 knockdown. Cells were grown for 14 days under treatment with or without 101 ng/mL Taxol for A2780 cells or 147 ng/mL Taxol for SKOV-3 cells and stained with crystal violet. The colonies were counted and captured. The data represent the mean ± SD from three independent experiments. *p < 0.05, and **p < 0.01

Article Snippet: Primary antibodies included a Rabbit Polyclonal antibody against human ST3GAL1 (LS-C185763, LifeSpan BioSciences) at a dilution of 1:1000.

Techniques: Concentration Assay, Colony Assay, Transfection, Over Expression, Transduction, Knockdown, Staining

Journal: Cell Death & Disease

Article Title: Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer

doi: 10.1038/s41419-018-1101-0

Figure Lengend Snippet: a The tumor growth curve of overexpression ST3GAL1 A2780 cells was compared with vector-expressing cells after Taxol treatment. Tumor growth was assessed in nude mice that were subcutaneously injected in the right flank with 2.0 × 106 stable transfectants. b, c Average tumor weight was measured and growth curves were generated (N = 6, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA). d Ki67 expression in the four groups was detected by immunohistochemistry, and TUNEL analysis in the four groups

Article Snippet: Primary antibodies included a Rabbit Polyclonal antibody against human ST3GAL1 (LS-C185763, LifeSpan BioSciences) at a dilution of 1:1000.

Techniques: Over Expression, Plasmid Preparation, Expressing, Injection, Generated, Immunohistochemistry, TUNEL Assay